Immunofluorescence analysis of hnRNP UL1 Monoclonal Antibody (Product # 14-9806-82) was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 30 minutes at 37°C, and blocked with 10% Normal Goat Serum for 1 hour at room temperature. The cells were labeled with 1.25 µg/mL of hnRNP UL1 Monoclonal Antibody (Product # 14-9806-82) in 0.1% Triton X-100, incubated overnight at 4°C and then labeled with 4 µg/mL of Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 conjugate (Product # A11006) for 1 hour at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with Fluoromount G with DAPI (Product # 00-4959). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents a merged image of control cells incubated with 1.25 µg/mL of Rat IgG2b Isotype Control (Product # 14-4031-85) antibody to assess background. The images were captured at 40X magnification.